Biofuels Lipase Mediated Synthesis Of Fatty Acid Esters

Lipase-Mediated Synthesis of Fatty Acid Esters for Use as Biofuels

Product Description

Lipase Super Y is a compound enzyme.

Microorganisms that generate lipase enzyme activity are therefore frequently exploited in the bioremediation of polluted environments in oil. Bacteria which degrade hydrocarbons are omnipresent in nature and use these compounds as rich sources of carbon and energy. Bioremediation has become very significant in removing the harmful contaminants from soil, water, or atmosphere in recent days.

For biodiesel, the enzymes lipase and phospholipase are the major players. A few companies are commercializing biodiesel produced with enzymatic processes. Lipase converts the free fatty acids (FFA) and triacylglycerol to fatty acid methyl esters—the main product comprising biodiesel. The phospholipase is responsible for converting phospholipids to diacylglycerol, which becomes a substrate for the lipase. Conventional processes using methanol and catalysts must remove the FFAs and the phospholipids prior to reactions to improve the quality of the biodiesel. Because the enzymes can utilize these as substrates, the yield is higher and the process saves chemical waste. Enzymes are known and available but the cost is often too high for them to be used on all feedstocks, particularly clean plant oils. One way of extending the life and thus lowering the cost of the enzymes is to immobilize them on a solid substrate to enable multiple cycles of use. Another solution is to produce them in a more cost efficient system or to improve their activity.

Features and Benefits

Lipase-Mediated Synthesis of Fatty Acid Esters for Use as Biofuels

Process

1. Feedstock Preparation: The first step in the process is to prepare the feedstock, which may be vegetable oil, animal fat, or another type of lipid. The feedstock must be cleaned and refined to remove any impurities and ensure consistency.

2. Enzyme Addition: Once the feedstock is prepared, lipase enzymes are added to the reaction mixture. The enzymes catalyze the transesterification reaction, which converts the triglycerides in the feedstock into fatty acid methyl esters (FAMEs) and glycerol.

3. Reaction Conditions: Depending on the specific enzyme and reaction conditions used, the reaction may take place under mild conditions, including lower temperatures and atmospheric pressure, reducing the overall energy requirements for the process.

4. Separation and Purification: After the reaction is complete, the FAMEs are separated from the glycerol and other byproducts using various separation techniques, such as centrifugation or distillation. The FAMEs are then purified to remove any remaining impurities and improve the quality of the final product.

5. Product Testing: Finally, the biodiesel product is tested to ensure that it meets industry standards and customer specifications. This may include testing for properties such as viscosity, flash point, and oxidative stability.

Who is KDN Biotech?

KDN Biotech is the subsidiary company of Vland Group, one of the largest manufacturers for enzymes.

KDN Biotech's product portfolio includes a wide range of enzymes, including proteases, lipases, cellulase, amylase and specialty enzymes.Below is a brief introduction:

1. Established in 2005. 1500+ employees. Vland listed in Shanghai Stock market in 2019, stock code 603739.

2. Detergent enzyme, bioenergy enzyme, textile enzyme, pulp & paper enzyme, feed enzyme, food enzyme etc.

3. 12 production bases, 8 technical centers, 2 laboratories, 32 Ph.Ds and about 300 Masters.

4. Customize products. Provide OEM/ODM service..

Our Production Scale and Capacity

• The factory covers a total area of 120 Chinese arcs, with a facility area of 60,000 m²
• Fermentation facility: The total area is 12,000m², including complete sets of three-stage fermentation, pilot scale-up fermenters, and automatic control system
• Recovery facility: with a total area of 20,000m² contain cell separation, UF concentration, formulation, mixtureation and spray dry
• Fermentation capacity: 1200 m³
• Liquid products: 5000 tons/year
• Powder products: 35000 tons/year